Human proinsulin standards
نویسندگان
چکیده
منابع مشابه
Human serum proinsulin.
Gel filtration of human serum extracts on Bio-Gel P-30 columns produced two peaks of material reactive with insulin antisera. The earlier eluting fraction appeared at the elution position of proinsulin (serum proinsulin-like component, PLC) while the second fraction corresponded in elution volume to insulin. In assays using porcine insulin-(131)I and an antiserum against porcine insulin, human ...
متن کاملKinetics of proinsulin conversion in human islets.
Islets isolated from human cadaver pancreas were pulse-labeled (10 min with [3H]leucine) and then incubated for a 180-min chase. Islets and chase medium were collected every 15 min and analyzed by reversed-phase HPLC to quantify the percentage of radioactively labeled proinsulin, conversion intermediates, and fully processed insulin. Release of proinsulin-related labeled products into the chase...
متن کاملFirst direct assay for intact human proinsulin.
We describe a sensitive two-site sandwich enzyme-linked immunosorbent assay for the measurement of intact human proinsulin in 100 microL of serum or plasma. The assay is based on the use of two monoclonal antibodies specific for epitopes at the C-peptide/insulin A chain junction and at the insulin B chain/C-peptide junction, respectively. Cross-reactivities with insulin, C-peptide, and the four...
متن کاملSpecific binding of proinsulin C-peptide to human cell membranes.
Recent reports have demonstrated beneficial effects of proinsulin C-peptide in the diabetic state, including improvements of kidney and nerve function. To examine the background to these effects, C-peptide binding to cell membranes has been studied by using fluorescence correlation spectroscopy. Measurements of ligand-membrane interactions at single-molecule detection sensitivity in 0.2-fl conf...
متن کاملHuman-Computer Interaction Standards
It is often assumed that a standard means a precise specification. Such standards have brought benefits in many fields, eg: bolts which screw into nuts, ATMs which can read credit cards, and compilers which can read programming languages. Some HCI standards are also of this type: many design guides provide a detailed specification of the nature of the user interface. Although standard user inte...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1984
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf00304860